RNA Extraction
RNA Extraction is a basic but important laboratory method used to get genetic material (specifically RNA) from cells or tissues. Think of it like separating egg yolk from egg white, but at a microscopic level. Lab technicians and scientists use this technique to study diseases, develop medical treatments, or check the quality of biological samples. This process is also sometimes called "RNA isolation" or "RNA purification." It's a fundamental skill in many biology and medical research labs, similar to how knowing how to use a microscope or maintain sterile conditions are basic requirements.
Examples in Resumes
Performed RNA Extraction and analysis on over 500 patient samples
Optimized RNA Isolation protocols resulting in 30% higher yield
Trained junior staff in RNA Purification techniques and safety procedures
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Online Communities
Job Resources
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot poor RNA yield in extractions?
Expected Answer: A senior technician should discuss checking sample storage conditions, reagent quality, possible contamination sources, and suggest multiple solution approaches while mentioning the importance of maintaining sample integrity.
Q: How would you train new staff in RNA extraction procedures?
Expected Answer: Should explain creating clear protocols, emphasizing safety procedures, quality control steps, and how to verify successful extractions. Should mention common mistakes to watch for and documentation requirements.
Mid Level Questions
Q: What safety precautions do you take when performing RNA extractions?
Expected Answer: Should mention proper protective equipment, handling of hazardous chemicals, proper disposal procedures, and contamination prevention measures.
Q: How do you ensure the quality of extracted RNA?
Expected Answer: Should discuss basic quality control methods, storage requirements, and how to check if the RNA is usable for further testing.
Junior Level Questions
Q: What are the basic steps of RNA extraction?
Expected Answer: Should be able to describe the main steps in simple terms: sample preparation, cell breaking, separating RNA from other materials, and proper storage of the final product.
Q: Why is it important to keep samples cold during RNA extraction?
Expected Answer: Should explain that RNA can break down easily and cold temperatures help preserve it, like keeping food fresh in a refrigerator.
Experience Level Indicators
Junior (0-2 years)
- Basic laboratory safety procedures
- Following written protocols accurately
- Sample preparation and handling
- Basic equipment operation
Mid (2-5 years)
- Troubleshooting extraction problems
- Quality control procedures
- Equipment maintenance
- Protocol optimization
Senior (5+ years)
- Training and supervising junior staff
- Developing new protocols
- Quality assurance management
- Laboratory safety oversight
Red Flags to Watch For
- Lack of attention to detail in following protocols
- Poor understanding of contamination prevention
- Careless approach to safety procedures
- Unable to maintain organized documentation
Related Terms
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