PCR (Polymerase Chain Reaction) is a fundamental laboratory technique used to make copies of DNA samples. Think of it like a DNA copying machine that can turn a tiny amount of genetic material into millions of copies for testing. This technique is widely used in medical labs, research facilities, and diagnostic centers. It became especially well-known during the COVID-19 pandemic as the main testing method. When you see PCR mentioned in a resume, it shows that the candidate has experience with modern laboratory techniques and DNA analysis.
Performed over 1,000 PCR tests for COVID-19 diagnosis
Developed new PCR protocols for genetic testing
Supervised team of technicians in PCR and RT-PCR testing procedures
Maintained quality control standards for PCR laboratory operations
Typical job title: "PCR Technicians"
Also try searching for:
Q: How would you handle quality control issues in a PCR laboratory?
Expected Answer: A senior technician should discuss implementing quality control measures, troubleshooting contamination issues, maintaining proper documentation, and training staff on best practices.
Q: Describe your experience managing a PCR testing facility during high-volume periods.
Expected Answer: Should demonstrate leadership experience, workflow optimization, resource management, and ability to maintain accuracy under pressure.
Q: What steps do you take to prevent contamination in PCR testing?
Expected Answer: Should explain basic laboratory hygiene, separate pre and post-PCR areas, proper use of controls, and contamination prevention protocols.
Q: How do you ensure accurate PCR results?
Expected Answer: Should discuss proper sample handling, use of positive and negative controls, equipment calibration, and following standard operating procedures.
Q: What are the basic steps of PCR testing?
Expected Answer: Should be able to explain sample preparation, DNA extraction, amplification process, and result interpretation in simple terms.
Q: What safety procedures do you follow in a PCR lab?
Expected Answer: Should demonstrate knowledge of personal protective equipment, proper handling of biological materials, and basic laboratory safety protocols.