A Laminar Flow Hood is a special workspace used in laboratories to keep samples and experiments clean and free from contamination. Think of it like a high-tech workbench with a built-in air filter system. It works by creating a steady flow of super-clean air that protects whatever scientists are working on. These are essential in places like medical labs, pharmaceutical companies, and research facilities where keeping things sterile is crucial. They're also sometimes called "clean benches," "laminar flow cabinets," or "laminar flow workstations."
Performed cell culture procedures using Laminar Flow Hood following sterile techniques
Managed quality control testing under Laminar Flow Cabinet for pharmaceutical products
Conducted sterile procedures using Clean Bench and Laminar Flow Workstation in microbiology research
Typical job title: "Laboratory Technicians"
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Q: How would you train new staff on proper laminar flow hood usage and maintenance?
Expected Answer: A senior candidate should explain the importance of proper training protocols, including demonstrating correct cleaning procedures, air flow testing, UV light usage, and documentation requirements. They should emphasize safety protocols and quality control measures.
Q: What steps would you take if you noticed contamination issues despite using the laminar flow hood?
Expected Answer: They should describe a systematic approach to troubleshooting, including checking air flow patterns, HEPA filter integrity, cleaning protocols, and proper technique review. They should also mention documentation and reporting procedures.
Q: Explain the difference between a laminar flow hood and a biological safety cabinet.
Expected Answer: Should be able to explain that laminar flow hoods protect the product only, while biological safety cabinets protect both the product and the operator. Should mention when each type is appropriate to use.
Q: What are the key maintenance procedures for a laminar flow hood?
Expected Answer: Should discuss regular cleaning protocols, UV light maintenance, HEPA filter monitoring, and airflow testing. Should also mention documentation requirements and scheduling of professional certifications.
Q: What basic steps do you take before working in a laminar flow hood?
Expected Answer: Should mention proper hand washing, wearing appropriate PPE, cleaning the work surface with appropriate disinfectant, and allowing the air to flow for several minutes before starting work.
Q: How do you maintain a sterile environment while working in a laminar flow hood?
Expected Answer: Should describe basic sterile technique principles like working from clean to dirty, not blocking air flow, keeping movements slow and deliberate, and proper arrangement of materials.