qPCR
qPCR (quantitative Polymerase Chain Reaction) is a common laboratory technique used to measure DNA or genetic material in samples. Think of it like a highly accurate copying machine that can also count how many copies it makes. Laboratory professionals use this method to detect diseases, monitor treatment effectiveness, or conduct research. It's similar to regular PCR testing (which became well-known during COVID-19), but qPCR provides specific measurements rather than just a yes/no result. When you see this on a resume, it indicates the person has experience with precise laboratory testing and genetic analysis.
Examples in Resumes
Performed over 1000 qPCR and RT-qPCR analyses for cancer research studies
Developed new qPCR protocols for rapid virus detection
Trained junior staff in Real-Time PCR and qPCR techniques
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Job Boards
Online Communities
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot inconsistent qPCR results?
Expected Answer: A senior technician should discuss checking sample quality, proper controls, machine calibration, and reagent integrity. They should mention documentation procedures and how to train others in problem-solving.
Q: How do you ensure quality control in a high-throughput qPCR lab?
Expected Answer: Should explain setting up standard operating procedures, implementing controls, maintaining equipment, proper sample handling, and staff training protocols.
Mid Level Questions
Q: What steps do you take to prevent contamination in qPCR work?
Expected Answer: Should describe separate areas for sample prep and analysis, proper cleaning procedures, use of appropriate protective equipment, and correct handling of materials.
Q: How do you validate a new qPCR method?
Expected Answer: Should explain running controls, comparing to existing methods, checking accuracy and repeatability, and proper documentation of results.
Junior Level Questions
Q: What are the basic components needed for a qPCR reaction?
Expected Answer: Should list main ingredients like DNA sample, primers, reaction mix, and explain basic steps of the process in simple terms.
Q: How do you maintain proper documentation in the lab?
Expected Answer: Should discuss lab notebooks, digital record-keeping, following standard operating procedures, and importance of accurate documentation.
Experience Level Indicators
Junior (0-2 years)
- Basic lab safety and sterile technique
- Sample preparation and handling
- Running standard qPCR protocols
- Basic data recording and analysis
Mid (2-5 years)
- Troubleshooting common problems
- Method optimization
- Data analysis and interpretation
- Training junior staff
Senior (5+ years)
- Protocol development and validation
- Quality control management
- Complex data analysis
- Lab management and staff supervision
Red Flags to Watch For
- No understanding of contamination control
- Inability to explain basic lab safety protocols
- Lack of attention to detail in documentation
- No experience with quality control procedures
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