Recruiter's Glossary

Examples: Capillary Electrophoresis • GWAS • RNA-seq

PCR Techniques

Term from Genetic Research industry explained for recruiters

PCR (Polymerase Chain Reaction) Techniques are fundamental laboratory methods used to make copies of DNA samples for genetic research and testing. Think of it like a DNA copying machine - scientists use it to turn a tiny amount of genetic material into enough copies to study. It's similar to how you might use a photocopier to make multiple copies of an important document. This process is essential in medical research, genetic testing, and disease diagnosis. When you see this term in resumes, it indicates the candidate has hands-on laboratory experience with genetic material handling.

Examples in Resumes

Performed PCR Techniques and PCR Analysis for genetic disease research projects

Optimized PCR protocols to improve testing efficiency by 40%

Trained junior researchers in PCR Techniques and Real-Time PCR methods

Typical job title: "Molecular Biologists"

Also try searching for:

Research Scientist Laboratory Technician Molecular Biology Technologist Genetic Research Associate Clinical Laboratory Scientist Biochemist Biotechnology Research Associate

Where to Find Molecular Biologists

Professional Organizations

Job Boards

Professional Networks

Example Interview Questions

Senior Level Questions

Q: How would you troubleshoot failed PCR reactions in the lab?

Expected Answer: A senior scientist should explain systematic approach to problem-solving, including checking reagent quality, optimizing temperatures, and validating primers. They should mention experience training others in troubleshooting.

Q: How would you design a new PCR protocol for a novel gene target?

Expected Answer: Should demonstrate knowledge of protocol development, including primer design, temperature optimization, and validation methods. Should mention cost and efficiency considerations.

Mid Level Questions

Q: What are the key differences between standard PCR and real-time PCR?

Expected Answer: Should explain that real-time PCR allows monitoring of DNA copying as it happens, while standard PCR only shows end results. Should mention basic applications of each method.

Q: How do you ensure quality control in PCR experiments?

Expected Answer: Should discuss using positive and negative controls, maintaining clean work areas, and proper sample handling procedures.

Junior Level Questions

Q: What are the basic components needed for a PCR reaction?

Expected Answer: Should list main ingredients like DNA template, primers, nucleotides, and polymerase enzyme. Basic understanding of each component's role is expected.

Q: What safety procedures do you follow when performing PCR?

Expected Answer: Should mention proper protective equipment, clean technique, and basic lab safety protocols.