Gel Electrophoresis
Gel Electrophoresis is a standard laboratory method used to analyze and separate DNA, RNA, or proteins based on their size. Think of it like a molecular sieve that sorts biological molecules, similar to how a strainer separates pasta of different sizes. This technique is fundamental in genetic research, medical diagnostics, and forensic science. When you see this term on a resume, it indicates that the candidate has hands-on laboratory experience with analyzing genetic material. It's as essential to genetic research as a microscope is to biology.
Examples in Resumes
Performed Gel Electrophoresis analysis on over 200 DNA samples for genetic mutation studies
Optimized Gel Electrophoresis protocols reducing analysis time by 30%
Trained junior researchers in Gel Electrophoresis and Agarose Gel Electrophoresis techniques
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Online Communities
Job Resources
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot poor band resolution in gel electrophoresis?
Expected Answer: A senior technician should discuss various factors like buffer concentration, voltage settings, gel percentage, and sample preparation. They should also mention experience in training others and developing standard operating procedures.
Q: How do you ensure quality control in gel electrophoresis experiments?
Expected Answer: Should explain the importance of using controls, maintaining proper documentation, calibrating equipment, and following standard protocols. Should also mention experience in validating results and troubleshooting.
Mid Level Questions
Q: What safety procedures do you follow when performing gel electrophoresis?
Expected Answer: Should discuss proper handling of electrical equipment, use of protective equipment, safe disposal of materials, and awareness of chemical hazards.
Q: How do you determine the appropriate gel concentration for your samples?
Expected Answer: Should explain how sample size affects gel percentage choice, and demonstrate understanding of different applications requiring different concentrations.
Junior Level Questions
Q: What is the basic principle of gel electrophoresis?
Expected Answer: Should be able to explain in simple terms how molecules move through the gel based on size, and describe basic setup of the equipment.
Q: What are the main components needed to perform gel electrophoresis?
Expected Answer: Should list basic equipment and materials like gel, buffer, power supply, and loading dyes, showing familiarity with basic laboratory setup.
Experience Level Indicators
Junior (0-2 years)
- Basic gel preparation and running
- Sample loading and documentation
- Understanding of laboratory safety
- Basic result interpretation
Mid (2-5 years)
- Troubleshooting common issues
- Multiple gel type experience
- Protocol optimization
- Quality control procedures
Senior (5+ years)
- Advanced analysis techniques
- Method development
- Training and supervision
- Complex sample analysis
Red Flags to Watch For
- No hands-on laboratory experience
- Unfamiliarity with basic safety protocols
- Inability to explain simple experimental procedures
- No experience with documentation practices
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