HPLC
HPLC (High-Performance Liquid Chromatography) is an important laboratory technique used to separate, identify, and measure different components in mixtures. Think of it like a very sophisticated filtering system that helps scientists analyze things like drugs, food ingredients, or environmental samples. It's similar to how a coffee filter separates coffee from grounds, but much more precise and scientific. When you see HPLC on a resume, it means the person has experience using this type of analytical equipment, which is essential in many laboratories, especially in pharmaceutical, food safety, or environmental testing industries.
Examples in Resumes
Performed daily analysis of pharmaceutical samples using HPLC techniques
Maintained and calibrated HPLC systems for quality control testing
Led method development for new HPLC procedures in food safety analysis
Typical job title: "HPLC Analysts"
Also try searching for:
Where to Find HPLC Analysts
Professional Organizations
Job Boards
Professional Networks
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot poor peak resolution in HPLC analysis?
Expected Answer: A senior analyst should explain in simple terms the systematic approach to problem-solving, like checking mobile phase composition, column condition, and instrument parameters. They should mention documentation and training of junior staff.
Q: Describe your experience with method development and validation.
Expected Answer: Should discuss how they create and verify new testing procedures, ensure accuracy and reliability, and explain their experience leading projects and training others.
Mid Level Questions
Q: How do you ensure sample analysis quality?
Expected Answer: Should explain basics of quality control, like running standards, checking calibrations, and following standard operating procedures (SOPs).
Q: What safety procedures do you follow when handling samples?
Expected Answer: Should discuss proper handling of chemicals, use of safety equipment, and following laboratory safety protocols.
Junior Level Questions
Q: What basic maintenance do you perform on HPLC equipment?
Expected Answer: Should describe routine tasks like changing mobile phases, cleaning the system, and basic troubleshooting.
Q: How do you prepare samples for HPLC analysis?
Expected Answer: Should explain basic sample preparation steps like filtering, diluting, and proper labeling of samples.
Experience Level Indicators
Junior (0-2 years)
- Basic HPLC operation
- Sample preparation
- Data recording
- Following standard procedures
Mid (2-5 years)
- Method optimization
- Troubleshooting
- Quality control procedures
- Data analysis and interpretation
Senior (5+ years)
- Method development
- Project leadership
- Training and supervision
- Regulatory compliance
Red Flags to Watch For
- No hands-on laboratory experience
- Unfamiliar with basic laboratory safety protocols
- Cannot explain simple sample preparation procedures
- No knowledge of quality control measures
Related Terms
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