Recruiter's Glossary

Examples: Immunology • Digital Pathology • Analytical Measurement Range

RT-PCR

Term from Diagnostics industry explained for recruiters

RT-PCR (Real-Time Polymerase Chain Reaction) is a standard laboratory technique used to detect specific genetic material in samples. Think of it as a highly accurate copying machine that can find and measure tiny amounts of DNA or RNA, making it essential for diagnosing diseases, including COVID-19. It's like a magnifying glass that helps scientists and lab technicians see if a specific disease is present in a patient sample. This technique is widely used in medical laboratories, research facilities, and diagnostic centers. When you see this on a resume, it usually indicates that the candidate has experience with diagnostic testing and laboratory procedures.

Examples in Resumes

Performed over 1000 RT-PCR tests for COVID-19 diagnosis in clinical laboratory setting

Supervised daily RT-PCR and Real-Time PCR testing operations for infectious disease detection

Trained junior technicians in RT-PCR and qPCR protocols and quality control procedures

Typical job title: "Laboratory Technicians"

Also try searching for:

Medical Laboratory Technician Clinical Laboratory Scientist Molecular Diagnostics Technician Research Technician Diagnostic Laboratory Technologist Clinical Research Associate Laboratory Analyst

Where to Find Laboratory Technicians

Professional Organizations

Job Boards

Professional Networks

Example Interview Questions

Senior Level Questions

Q: How would you handle quality control issues in RT-PCR testing?

Expected Answer: A senior technician should explain the importance of positive/negative controls, maintaining proper documentation, equipment calibration, and steps to troubleshoot when results are questionable. They should also mention staff training and quality assurance protocols.

Q: Describe your experience with laboratory management and test validation.

Expected Answer: Should discuss experience with supervising staff, implementing new procedures, maintaining regulatory compliance, and validating new test methods. Should mention documentation and training processes.

Mid Level Questions

Q: What steps do you take to prevent contamination in RT-PCR testing?

Expected Answer: Should explain basic lab hygiene, separate areas for different steps, proper PPE usage, and cleaning procedures. Should also mention how to identify and handle potential contamination.

Q: How do you ensure accurate results in RT-PCR testing?

Expected Answer: Should discuss proper sample handling, running appropriate controls, following standard operating procedures, and proper documentation of results. Should mention what to do if results are unclear.

Junior Level Questions

Q: What safety procedures do you follow in the laboratory?

Expected Answer: Should be able to explain basic lab safety rules, proper PPE usage, handling of biological samples, and waste disposal procedures.

Q: Describe the basic steps of performing an RT-PCR test.

Expected Answer: Should be able to explain sample preparation, running the test, and basic result interpretation in simple terms. Should understand the importance of following protocols exactly.