Pipetting is a fundamental laboratory skill that involves accurately measuring and transferring small amounts of liquids using special tools called pipettes. This is similar to using an eye dropper, but with much more precision. Laboratory technicians use pipettes for many tasks, from preparing medical tests to conducting research experiments. Think of it as using a very precise measuring cup in a kitchen, but for tiny amounts of liquids that need to be exactly right. This skill is essential in medical labs, research facilities, and pharmaceutical companies.
Performed precise Pipetting techniques for DNA sample preparation
Trained junior staff in proper Pipetting and sample handling procedures
Maintained accuracy in Pipetting for high-throughput screening of clinical samples
Typical job title: "Laboratory Technicians"
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Q: How would you implement quality control measures for pipetting in a laboratory?
Expected Answer: A senior technician should discuss calibration procedures, regular accuracy checks, documentation methods, training programs for staff, and troubleshooting common pipetting errors.
Q: How do you handle high-throughput pipetting while maintaining accuracy?
Expected Answer: Should explain strategies for maintaining consistency during lengthy procedures, ergonomic considerations, quality control measures, and when to use automated systems versus manual pipetting.
Q: What are the main sources of pipetting errors and how do you prevent them?
Expected Answer: Should identify common issues like air bubbles, incorrect tip usage, and volume inconsistencies, and explain prevention methods and good pipetting techniques.
Q: Describe your experience with different types of pipettes and when to use each.
Expected Answer: Should discuss various pipette types (single-channel, multi-channel, electronic), their applications, and maintenance requirements.
Q: What are the basic steps for proper pipetting technique?
Expected Answer: Should describe proper posture, tip selection, pre-rinsing when needed, and consistent plunger pressure for accurate measurements.
Q: How do you maintain and clean pipettes?
Expected Answer: Should explain basic cleaning procedures, when to change tips, how to prevent contamination, and when to request calibration.