HPLC
HPLC (High-Performance Liquid Chromatography) is an important laboratory technique used to separate, identify, and measure different components in mixtures. Think of it like a very sophisticated filtering system that helps scientists ensure medications are pure and contain the right ingredients in the right amounts. It's one of the most commonly used tools in pharmaceutical labs, similar to how a chef needs an oven in the kitchen. When you see HPLC on a resume, it means the person has experience with quality testing and analyzing pharmaceutical products. You might also see it called "High-Pressure Liquid Chromatography" - it's the same thing.
Examples in Resumes
Performed quality control testing using HPLC analysis for over 200 pharmaceutical samples monthly
Led method development and validation of HPLC techniques for new drug products
Maintained and calibrated HPLC systems to ensure FDA compliance
Conducted analysis using High-Performance Liquid Chromatography for drug stability studies
Typical job title: "HPLC Analysts"
Also try searching for:
Where to Find HPLC Analysts
Professional Organizations
Job Boards
Professional Networks
Example Interview Questions
Senior Level Questions
Q: How would you approach troubleshooting an HPLC system that's giving inconsistent results?
Expected Answer: A senior analyst should discuss a systematic approach to problem-solving, including checking mobile phase quality, column condition, instrument calibration, and sample preparation methods. They should mention documentation requirements and team training aspects.
Q: Tell me about a time you developed and validated a new HPLC method.
Expected Answer: Should explain the process of creating a new testing method in simple terms, including how they ensured accuracy, got approval from regulators, and trained others to use it.
Mid Level Questions
Q: What steps do you take to maintain HPLC equipment?
Expected Answer: Should describe regular maintenance procedures, record-keeping practices, and basic troubleshooting skills. Should mention how they ensure the equipment stays accurate and reliable.
Q: How do you ensure the quality of your HPLC results?
Expected Answer: Should discuss quality control measures, standard operating procedures, and how they verify results are accurate and reproducible.
Junior Level Questions
Q: What safety precautions do you take when operating HPLC equipment?
Expected Answer: Should demonstrate basic knowledge of laboratory safety, handling of chemical materials, and proper operation of HPLC instruments.
Q: Can you explain the basic components of an HPLC system?
Expected Answer: Should be able to describe the main parts of the system in simple terms and explain their basic functions in the analysis process.
Experience Level Indicators
Junior (0-2 years)
- Basic HPLC operation and maintenance
- Sample preparation
- Data recording and basic analysis
- Following standard operating procedures
Mid (2-5 years)
- Method execution and optimization
- Equipment troubleshooting
- Quality control procedures
- Data interpretation and reporting
Senior (5+ years)
- Method development and validation
- Regulatory compliance management
- Team training and supervision
- Complex problem-solving
Red Flags to Watch For
- No hands-on experience with HPLC equipment
- Unfamiliarity with laboratory safety protocols
- Lack of understanding of quality control procedures
- No knowledge of regulatory requirements
- Poor documentation practices
Related Terms
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