ELISA
ELISA (Enzyme-Linked Immunosorbent Assay) is a common laboratory test used to detect and measure substances like proteins, antibodies, or hormones in biological samples. Think of it like a very precise detective tool that scientists use to find specific substances in blood, urine, or other samples. It's widely used in medical testing, research, and diagnostic labs. Similar tests include Western Blot or RIA (Radioimmunoassay), but ELISA is often preferred because it's safer, more cost-effective, and gives reliable results. When you see this on a resume, it means the person has experience with laboratory testing and analysis.
Examples in Resumes
Performed over 1000 ELISA tests for clinical trials
Developed and validated new ELISA protocols for protein detection
Trained junior staff in ELISA techniques and quality control procedures
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Online Communities
Job Resources
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot inconsistent ELISA results?
Expected Answer: A senior technician should discuss systematic approach including checking reagent quality, equipment calibration, temperature control, and proper washing techniques. They should mention documentation and quality control measures.
Q: How would you validate a new ELISA method?
Expected Answer: Should explain process of testing accuracy, precision, sensitivity, and reproducibility. Should mention importance of positive/negative controls and creating standard operating procedures.
Mid Level Questions
Q: What steps do you take to ensure quality control in ELISA testing?
Expected Answer: Should mention using standard curves, running duplicates, including proper controls, maintaining equipment, and following standard operating procedures.
Q: How do you handle and store ELISA reagents properly?
Expected Answer: Should discuss proper temperature storage, avoiding freeze-thaw cycles, maintaining inventory records, and following manufacturer guidelines for reagent handling.
Junior Level Questions
Q: What are the basic steps of an ELISA test?
Expected Answer: Should be able to describe the main steps: sample preparation, adding reagents, washing steps, adding substrate, and reading results.
Q: What safety precautions do you take when performing ELISA?
Expected Answer: Should mention wearing proper PPE (gloves, lab coat), handling biological samples safely, proper disposal of materials, and following laboratory safety protocols.
Experience Level Indicators
Junior (0-2 years)
- Basic laboratory techniques
- Following standard ELISA protocols
- Sample preparation
- Data recording and basic analysis
Mid (2-5 years)
- Troubleshooting common problems
- Quality control procedures
- Method optimization
- Training junior staff
Senior (5+ years)
- Protocol development and validation
- Complex data analysis
- Project management
- Equipment maintenance and calibration
Red Flags to Watch For
- No experience with laboratory safety protocols
- Unable to explain basic quality control measures
- Lack of attention to detail in procedure documentation
- No understanding of proper sample handling
