Electrophoresis is a common laboratory technique used to separate and analyze different types of molecules, most often proteins or DNA. Think of it like sorting items by size using an electric current - similar to how a conveyor belt moves items, but for tiny molecules. Lab technicians and scientists use this method to test samples, diagnose diseases, or perform research. When you see this term on a resume, it usually means the candidate has hands-on experience with laboratory equipment and can perform detailed analytical work. This skill is particularly valuable in medical labs, research facilities, and biotechnology companies.
Performed Electrophoresis analysis on over 500 patient samples monthly
Trained junior staff in Gel Electrophoresis techniques and safety protocols
Optimized Protein Electrophoresis procedures reducing analysis time by 25%
Typical job title: "Laboratory Technicians"
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Q: How would you troubleshoot inconsistent electrophoresis results?
Expected Answer: A senior technician should discuss systematic approaches to problem-solving, including checking buffer conditions, power settings, sample preparation methods, and equipment maintenance. They should mention documentation and training implications.
Q: How do you ensure quality control in an electrophoresis laboratory?
Expected Answer: Should explain standard operating procedures, calibration protocols, use of controls, documentation requirements, and staff training approaches. Should also mention regulatory compliance and safety measures.
Q: What safety measures do you follow when performing electrophoresis?
Expected Answer: Should discuss proper handling of electrical equipment, use of protective gear, proper disposal of materials, and following standard safety protocols. Should also mention documentation and reporting procedures.
Q: How do you maintain accurate records of electrophoresis results?
Expected Answer: Should explain laboratory information management systems (LIMS), proper documentation practices, data backup procedures, and quality control measures.
Q: What is the basic principle of electrophoresis?
Expected Answer: Should explain in simple terms how molecules move through a gel using electrical current, and basic sample preparation steps. Should demonstrate understanding of safety procedures.
Q: What types of samples can be analyzed using electrophoresis?
Expected Answer: Should mention common sample types like proteins, DNA, and RNA, and basic preparation methods. Should show understanding of different gel types and their uses.