Centrifugation
Centrifugation is a common laboratory technique used to separate different materials in a sample, like separating blood components or purifying substances. Think of it like a super-fast spinning machine that uses circular motion (like a washing machine's spin cycle) to separate things based on their weight. Lab professionals use this method daily for testing, research, and sample preparation. The equipment used is called a centrifuge, and it comes in different sizes and types. This skill is essential in medical labs, research facilities, and industrial laboratories.
Examples in Resumes
Performed daily sample preparation using Centrifugation techniques for blood analysis
Operated and maintained various Centrifuge systems for DNA extraction
Trained junior staff in proper Centrifugation protocols and safety procedures
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Job Boards
Online Communities
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot if a centrifuge is not properly separating samples?
Expected Answer: A senior technician should discuss checking speed settings, balance, temperature, sample preparation methods, and equipment maintenance history. They should also mention documentation and safety protocols.
Q: How do you ensure quality control in centrifugation procedures?
Expected Answer: Should explain monitoring speed and time settings, proper sample handling, regular calibration, maintaining logs, and training other staff members on proper procedures.
Mid Level Questions
Q: What safety precautions do you take when operating a centrifuge?
Expected Answer: Should mention proper balance of samples, checking for tube integrity, using appropriate safety equipment, following speed limits, and proper cleanup procedures.
Q: How do you determine the right speed and time for centrifugation?
Expected Answer: Should explain following standard protocols, considering sample type, desired separation, and understanding the relationship between speed and separation quality.
Junior Level Questions
Q: What is the basic principle of centrifugation?
Expected Answer: Should explain that it's a process of spinning samples at high speeds to separate materials based on their weight, like separating blood components or purifying samples.
Q: How do you properly balance samples in a centrifuge?
Expected Answer: Should describe placing tubes of equal weight opposite each other, using balance tubes when necessary, and checking for proper tube placement.
Experience Level Indicators
Junior (0-2 years)
- Basic centrifuge operation
- Sample preparation
- Safety protocols
- Basic maintenance procedures
Mid (2-5 years)
- Advanced sample processing
- Equipment troubleshooting
- Protocol optimization
- Training junior staff
Senior (5+ years)
- Complex separation techniques
- Quality control management
- Protocol development
- Equipment validation and maintenance
Red Flags to Watch For
- Unfamiliarity with basic safety protocols
- No experience with sample preparation
- Lack of knowledge about contamination prevention
- Poor understanding of documentation requirements
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