Centrifugation
Centrifugation is a standard laboratory method used to separate different components in liquids, similar to how a washing machine spins to remove water from clothes. In biotech labs, scientists use centrifuges (special spinning machines) to separate cells, proteins, DNA, or other biological materials. This technique is fundamental in many biotech processes, from developing medicines to testing samples. When you see this term in a resume, it typically indicates the candidate has hands-on laboratory experience. Related terms you might see include "separation techniques" or "sample preparation."
Examples in Resumes
Performed Centrifugation techniques to isolate protein samples for analysis
Optimized Centrifugation protocols for cell separation
Trained junior staff in proper Centrifugation methods and safety procedures
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Job Boards
Professional Networks
Example Interview Questions
Senior Level Questions
Q: How would you optimize a centrifugation protocol for a new product?
Expected Answer: A senior scientist should discuss factors like speed, time, temperature, and sample preparation. They should mention previous experience in protocol development and troubleshooting.
Q: Describe a time when you improved a centrifugation-based process.
Expected Answer: Should demonstrate leadership in process improvement, cost reduction, or efficiency gains, with specific examples of changes made and results achieved.
Mid Level Questions
Q: What safety considerations are important when using centrifuges?
Expected Answer: Should discuss proper balance, speed limits, sample containment, and emergency procedures. Should show awareness of both personal and sample safety.
Q: How do you determine the right centrifugation settings for different samples?
Expected Answer: Should explain basic principles of selecting speed and time based on sample type, and demonstrate knowledge of common protocols.
Junior Level Questions
Q: What is the basic principle of centrifugation?
Expected Answer: Should explain in simple terms how spinning separates materials based on their weight, like a washing machine separating water from clothes.
Q: How do you properly balance a centrifuge?
Expected Answer: Should describe basic steps of sample balancing, use of counter-weights, and why balance is important for safety and results.
Experience Level Indicators
Junior (0-2 years)
- Basic centrifuge operation
- Sample preparation
- Safety protocols
- Record keeping
Mid (2-5 years)
- Protocol optimization
- Multiple centrifuge types operation
- Troubleshooting
- Training others
Senior (5+ years)
- Process development
- Method validation
- Team supervision
- SOP writing
Red Flags to Watch For
- No understanding of basic lab safety
- Inability to explain sample preparation steps
- No experience with documentation
- Lack of attention to detail in protocols
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