ELISA
ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used laboratory test in medical and research settings. Think of it as a sophisticated detection tool that helps identify specific substances in blood samples or other biological materials. It's like a highly accurate "search and find" method that scientists use to detect things like diseases, hormones, or other biological markers. When you see ELISA mentioned in a resume, it means the candidate has experience with this important testing method that's commonly used in clinical laboratories, research facilities, and diagnostic centers.
Examples in Resumes
Performed over 1,000 ELISA tests for clinical trials
Developed and validated new ELISA protocols for protein detection
Trained junior staff in ELISA techniques and quality control procedures
Conducted high-throughput ELISA assays for diagnostic testing
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Online Communities
Job Resources
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot inconsistent ELISA results?
Expected Answer: A senior technician should discuss systematic approaches like checking reagent quality, equipment calibration, temperature control, and proper washing techniques. They should also mention documentation and quality control measures.
Q: How would you develop and validate a new ELISA protocol?
Expected Answer: Should explain the process of optimization, including antibody selection, determining optimal concentrations, establishing standard curves, and validation steps including reproducibility testing.
Mid Level Questions
Q: What quality control measures do you use when running ELISA tests?
Expected Answer: Should discuss using positive and negative controls, standard curves, duplicate samples, and proper documentation of results and calibration records.
Q: Explain the difference between direct and indirect ELISA.
Expected Answer: Should be able to explain in simple terms how these two methods differ and when each would be more appropriate to use.
Junior Level Questions
Q: What are the basic steps of an ELISA test?
Expected Answer: Should be able to describe the main steps: sample preparation, coating, blocking, adding samples and antibodies, washing, and reading results.
Q: How do you maintain accurate records of ELISA results?
Expected Answer: Should discuss basic lab notebook keeping, data recording, and using standard forms or electronic systems to track results.
Experience Level Indicators
Junior (0-2 years)
- Basic ELISA procedures
- Laboratory safety protocols
- Sample preparation
- Data recording and documentation
Mid (2-5 years)
- Troubleshooting techniques
- Quality control procedures
- Method optimization
- Equipment maintenance
Senior (5+ years)
- Protocol development and validation
- Training and supervision
- Complex problem solving
- Quality assurance management
Red Flags to Watch For
- No hands-on laboratory experience
- Unfamiliarity with basic lab safety protocols
- Poor attention to detail in procedure documentation
- Lack of understanding of quality control importance
