Recombinant DNA is a key laboratory technique used to combine genetic material from different sources to create something new and useful. Think of it like a genetic copy-and-paste tool that scientists use to make medicines, improve crops, or create research materials. When you see this term on a resume, it usually means the person has experience working in genetic engineering labs. Similar terms you might see are "genetic engineering," "molecular cloning," or "DNA manipulation." This is a fundamental skill in biotechnology, especially in developing new medicines or improving agricultural products.
Developed new research protocols using Recombinant DNA techniques to produce therapeutic proteins
Led team of 3 scientists in Recombinant DNA and DNA Engineering projects for vaccine development
Optimized Recombinant DNA processes resulting in 40% increased protein production efficiency
Typical job title: "Molecular Biologists"
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Q: How would you design a strategy to optimize protein expression in a recombinant system?
Expected Answer: A senior scientist should discuss different expression systems (bacterial, mammalian, etc.), optimization techniques, and troubleshooting approaches. They should mention factors like codon optimization, promoter selection, and scale-up considerations.
Q: What experience do you have in leading recombinant DNA projects and managing teams?
Expected Answer: Look for answers that demonstrate project management skills, experience in coordinating multiple experiments, mentoring junior scientists, and successful completion of major research projects.
Q: What methods do you use to verify successful DNA recombination?
Expected Answer: Candidate should be able to explain common verification methods in simple terms, such as DNA sequencing, gel analysis, and functional testing of the final product.
Q: Describe a challenging recombinant DNA project you worked on and how you solved problems.
Expected Answer: Look for practical problem-solving examples, ability to troubleshoot technical issues, and systematic approach to resolving experimental challenges.
Q: What basic lab safety protocols are important when working with recombinant DNA?
Expected Answer: Should demonstrate understanding of basic laboratory safety, containment levels, proper handling of biological materials, and awareness of regulatory requirements.
Q: Can you explain the basic steps of a recombinant DNA experiment?
Expected Answer: Should be able to describe the fundamental steps like DNA isolation, cutting DNA, joining DNA pieces together, and introducing DNA into host cells in simple terms.