qPCR
qPCR (also known as Real-Time PCR) is a laboratory technique used to measure and analyze DNA in samples. Think of it like a DNA photocopier that not only makes copies but also counts them in real-time. Scientists use this method to detect diseases, study gene expression, or verify the presence of specific genetic material. It's an improved version of regular PCR (Polymerase Chain Reaction), with the 'q' standing for 'quantitative' because it can tell exactly how much DNA is present. This skill is essential in medical labs, research facilities, and biotech companies.
Examples in Resumes
Performed qPCR analysis on over 500 patient samples for COVID-19 testing
Developed standardized protocols for Real-Time PCR and trained junior staff
Utilized qPCR techniques to study gene expression in cancer research
Conducted high-throughput RT-PCR screening for genetic disorders
Typical job title: "Molecular Biology Technicians"
Also try searching for:
Where to Find Molecular Biology Technicians
Professional Organizations
Online Communities
Job Resources
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot inconsistent qPCR results?
Expected Answer: A senior should discuss systematic approaches like checking sample quality, primer design, instrument calibration, and proper controls. They should mention experience training others in problem-solving these issues.
Q: How do you validate and implement new qPCR methods in the lab?
Expected Answer: Should explain the process of method validation, including accuracy testing, precision measurements, and creating standard operating procedures. Should discuss training staff and ensuring quality control.
Mid Level Questions
Q: What factors are important when designing a qPCR experiment?
Expected Answer: Should mention sample preparation, proper controls, primer selection, and basic troubleshooting. Should understand the importance of replicates and standardization.
Q: How do you ensure quality control in qPCR experiments?
Expected Answer: Should discuss using proper controls, maintaining clean workspace, regular calibration, and following standard protocols. Should understand basic troubleshooting steps.
Junior Level Questions
Q: What safety measures do you follow when performing qPCR?
Expected Answer: Should discuss basic lab safety, proper handling of biological samples, use of PPE, and following standard protocols.
Q: Can you explain the basic steps of a qPCR experiment?
Expected Answer: Should be able to describe sample preparation, adding reagents, running the machine, and basic data collection. Should understand the importance of following protocols exactly.
Experience Level Indicators
Junior (0-2 years)
- Basic lab safety and sterile technique
- Sample preparation and handling
- Following established protocols
- Basic data collection and recording
Mid (2-5 years)
- Independent experiment design
- Troubleshooting common issues
- Data analysis and interpretation
- Method optimization
Senior (5+ years)
- Protocol development and validation
- Training and supervising junior staff
- Complex experimental design
- Quality control management
Red Flags to Watch For
- Unfamiliarity with basic lab safety protocols
- No experience with sample preparation or handling
- Unable to explain basic PCR concepts
- Poor documentation practices
- No understanding of contamination prevention
Related Terms
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