PCR
PCR (Polymerase Chain Reaction) is a fundamental laboratory technique used to analyze and detect genetic material. Think of it as a photocopier for DNA - it can make millions of copies from a tiny sample, making it easier to study and detect. This technique is widely used in medical diagnostics, especially for detecting infections, genetic conditions, and during the COVID-19 pandemic. When you see PCR mentioned in a resume, it usually means the candidate has experience with diagnostic testing and laboratory procedures. Similar terms you might see include RT-PCR (Real-Time PCR) or qPCR (Quantitative PCR), which are more specific versions of the same basic technique.
Examples in Resumes
Performed over 1,000 PCR tests for COVID-19 diagnosis in clinical laboratory setting
Supervised team of technicians conducting PCR and RT-PCR testing procedures
Developed quality control protocols for PCR and molecular diagnostic testing
Typical job title: "PCR Technicians"
Also try searching for:
Where to Find PCR Technicians
Professional Organizations
Job Boards
Professional Networks
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot failed PCR reactions in the laboratory?
Expected Answer: A senior technician should explain systematic approach to identifying issues, from checking reagent quality to examining temperature settings, and mention documentation procedures and quality control measures.
Q: Describe your experience training others in PCR techniques.
Expected Answer: Should discuss creating training protocols, ensuring consistent results across team members, and maintaining quality standards while teaching others.
Mid Level Questions
Q: What quality control measures do you use when running PCR tests?
Expected Answer: Should mention using positive and negative controls, maintaining proper documentation, and following standard operating procedures for accurate results.
Q: How do you ensure sample integrity in PCR testing?
Expected Answer: Should discuss proper sample handling, storage conditions, contamination prevention, and documentation procedures.
Junior Level Questions
Q: What are the basic steps of PCR testing?
Expected Answer: Should be able to explain sample preparation, the basic process of DNA multiplication, and result interpretation in simple terms.
Q: What safety procedures do you follow when handling laboratory samples?
Expected Answer: Should demonstrate knowledge of basic laboratory safety, proper protective equipment use, and handling of biological materials.
Experience Level Indicators
Junior (0-2 years)
- Basic PCR testing procedures
- Laboratory safety protocols
- Sample preparation
- Basic documentation practices
Mid (2-5 years)
- Advanced testing techniques
- Quality control procedures
- Equipment maintenance
- Results interpretation
Senior (5+ years)
- Method development
- Staff training and supervision
- Troubleshooting complex issues
- Quality assurance management
Red Flags to Watch For
- No experience with laboratory safety protocols
- Lack of attention to detail in documentation
- Unable to explain basic PCR concepts
- No understanding of quality control measures
Related Terms
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