NGS (Next Generation Sequencing) is a modern way to analyze DNA and genetic information in medical and research settings. Think of it like a very advanced reading machine that can quickly read through large amounts of genetic code to help doctors and researchers understand diseases, predict health risks, and choose the right treatments for patients. It's much faster and more detailed than older methods of genetic testing. When you see this term in resumes, it usually means the person has experience with these advanced genetic testing technologies and the related computer systems used to process the results.
Performed NGS analysis for over 500 patient samples in clinical diagnostics setting
Led implementation of new Next Generation Sequencing protocols in the laboratory
Managed NGS and Next-Gen Sequencing data analysis pipeline for cancer research studies
Typical job title: "NGS Technologists"
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Q: How would you handle quality control issues in NGS runs?
Expected Answer: A senior technologist should discuss systematic approaches to troubleshooting, including sample quality assessment, library preparation checks, and instrument maintenance protocols. They should also mention documentation and team training aspects.
Q: How do you stay current with NGS technology developments?
Expected Answer: Should demonstrate knowledge of professional development through conferences, workshops, vendor training, and keeping up with new protocols and instruments in the field.
Q: What steps do you take to ensure sample quality for NGS?
Expected Answer: Should explain basic sample handling procedures, quality checks, and common issues that can affect sequencing results. Should mention documentation and tracking systems.
Q: How do you handle multiple urgent samples in your workflow?
Expected Answer: Should discuss prioritization strategies, communication with stakeholders, and maintaining quality while managing time pressure.
Q: What safety procedures are important when handling NGS samples?
Expected Answer: Should demonstrate understanding of basic laboratory safety, proper sample handling, and contamination prevention procedures.
Q: Describe the basic steps of an NGS workflow.
Expected Answer: Should be able to explain the main steps from sample receipt to final report in simple terms, showing understanding of the overall process.