ELISA
ELISA (Enzyme-Linked Immunosorbent Assay) is a commonly used laboratory test that helps detect substances in blood samples and other biological materials. Think of it like a very precise detective tool that scientists and lab technicians use to find specific proteins, antibodies, or other molecules. It's widely used in medical diagnostics, research, and food safety testing. When you see this term in a resume, it usually means the candidate has experience running these types of tests in a laboratory setting. This skill is particularly important in clinical laboratories, research facilities, and diagnostic companies.
Examples in Resumes
Performed over 1,000 ELISA tests for COVID-19 antibody detection
Developed and validated new ELISA protocols for food allergen testing
Supervised junior technicians in running ELISA and EIA assays
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Job Boards
Professional Networks
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot inconsistent ELISA results?
Expected Answer: A senior technician should discuss checking reagent quality, temperature control, washing procedures, and plate reader calibration. They should also mention training others and implementing quality control measures.
Q: How do you ensure quality control in an ELISA testing laboratory?
Expected Answer: Should explain standard operating procedures, control samples, documentation requirements, and how to train staff on proper techniques and maintaining testing accuracy.
Mid Level Questions
Q: What steps do you take to validate an ELISA test?
Expected Answer: Should describe running positive and negative controls, checking for cross-reactivity, and ensuring consistent results across multiple runs.
Q: How do you handle multiple ELISA test requests in a time-sensitive environment?
Expected Answer: Should discuss prioritizing samples, organizing workflow, maintaining accuracy while working quickly, and coordinating with team members.
Junior Level Questions
Q: What are the basic steps of an ELISA test?
Expected Answer: Should be able to describe the main steps: sample preparation, adding reagents, washing, and reading results. Basic understanding of why each step is important.
Q: How do you maintain proper documentation for ELISA tests?
Expected Answer: Should explain recording test results, maintaining reagent logs, and following laboratory documentation procedures.
Experience Level Indicators
Junior (0-2 years)
- Basic ELISA procedures
- Laboratory safety protocols
- Sample preparation
- Results recording and documentation
Mid (2-5 years)
- Multiple ELISA test types
- Quality control procedures
- Equipment maintenance
- Test optimization
Senior (5+ years)
- Protocol development
- Staff training and supervision
- Troubleshooting complex issues
- Quality assurance management
Red Flags to Watch For
- No hands-on laboratory experience
- Unfamiliarity with laboratory safety protocols
- Poor attention to detail in documentation
- Lack of understanding of quality control procedures
