Chromatography is a key laboratory technique used to separate and analyze different components in mixtures, which is essential in pharmaceutical and chemical industries. Think of it like a sorting system that helps scientists identify what ingredients are in a sample, measure their amounts, and ensure product quality. This technique is widely used in drug development, quality control, and research. Common types include HPLC (High Performance Liquid Chromatography) and GC (Gas Chromatography), which are like different methods of sorting, each best suited for specific types of materials.
Performed quality control testing using Chromatography and HPLC techniques
Supervised Chromatography lab operations for drug development projects
Validated Chromatographic methods for new pharmaceutical products
Typical job title: "Chromatography Analysts"
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Q: How would you approach method development for a new drug compound?
Expected Answer: A senior analyst should explain the step-by-step process of developing a testing method, including considerations for sample preparation, choosing the right type of chromatography, and how to ensure the method is reliable and reproducible.
Q: How do you handle method validation failures?
Expected Answer: Should discuss troubleshooting approaches, understanding root causes, documentation requirements, and the process of adjusting methods while maintaining compliance with industry standards.
Q: What quality control measures do you use when running samples?
Expected Answer: Should mention system suitability tests, calibration standards, quality control samples, and basic troubleshooting of common issues.
Q: How do you ensure data integrity in your analytical work?
Expected Answer: Should explain proper documentation practices, following SOPs, data backup procedures, and maintaining sample tracking records.
Q: Can you explain basic sample preparation for chromatography analysis?
Expected Answer: Should be able to describe simple steps like filtration, dilution, and basic handling of samples before analysis.
Q: What safety procedures do you follow in the laboratory?
Expected Answer: Should demonstrate knowledge of basic lab safety, proper handling of chemicals, and use of personal protective equipment.