Chromatography is a widely used laboratory method for separating and analyzing mixtures of chemicals. Think of it like a sophisticated sorting system - similar to how a coffee filter separates coffee from grounds, but much more precise. Scientists and lab technicians use this technique in many industries, from drug testing and environmental analysis to food safety and pharmaceutical development. There are different types, like gas chromatography (GC) and liquid chromatography (LC), but they all serve the same basic purpose: helping identify what substances are in a sample and how much of each is present.
Performed quality control testing using Chromatography techniques on pharmaceutical products
Operated Gas Chromatography and Liquid Chromatography systems for environmental sample analysis
Maintained and calibrated Chromatography equipment for food safety testing
Typical job title: "Chromatography Technicians"
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Q: How would you troubleshoot unexpected results in a chromatography analysis?
Expected Answer: A senior analyst should explain systematic approach to problem-solving: checking instrument calibration, sample preparation methods, column condition, and mobile phase composition. They should mention documentation and communication with team members.
Q: How do you ensure quality control in a chromatography laboratory?
Expected Answer: Should discuss standard operating procedures, calibration protocols, documentation practices, and how to train junior staff. Should mention regulatory compliance and quality assurance measures.
Q: What factors affect the separation of compounds in chromatography?
Expected Answer: Should explain basic concepts like mobile phase composition, temperature, flow rate, and column selection in simple terms. Should demonstrate understanding of method optimization.
Q: How do you maintain chromatography equipment?
Expected Answer: Should describe routine maintenance procedures, cleaning protocols, and basic troubleshooting. Should mention record-keeping and when to call for service support.
Q: What safety procedures do you follow when handling chemical samples?
Expected Answer: Should mention proper PPE use, understanding of Safety Data Sheets, proper waste disposal, and basic laboratory safety protocols.
Q: How do you prepare samples for chromatography analysis?
Expected Answer: Should describe basic sample preparation steps like filtration, dilution, and proper labeling. Should understand importance of avoiding contamination.