Centrifugation
Centrifugation is a common laboratory technique used to separate different components in a mixture based on their weight. Think of it like a super-fast spinning machine that helps separate materials, similar to how a washing machine spins to remove water from clothes. Lab technicians and scientists use this process to separate blood components, analyze samples, or purify substances. The equipment used is called a centrifuge, and knowing how to operate different types of centrifuges is an important skill in medical labs, research facilities, and diagnostic centers.
Examples in Resumes
Performed daily sample preparation using Centrifugation techniques for blood analysis
Operated high-speed Centrifuge equipment for DNA extraction procedures
Trained junior staff on proper Centrifugation protocols and safety measures
Typical job title: "Laboratory Technicians"
Also try searching for:
Where to Find Laboratory Technicians
Professional Organizations
Job Boards
Professional Networks
Example Interview Questions
Senior Level Questions
Q: How would you troubleshoot if samples are not separating properly during centrifugation?
Expected Answer: A senior technician should discuss checking speed settings, balance, temperature, time settings, and sample preparation methods. They should also mention documentation and maintenance procedures.
Q: How do you ensure quality control in centrifugation procedures?
Expected Answer: Should explain regular calibration checks, temperature monitoring, maintenance schedules, proper documentation, and training of other staff members on standard operating procedures.
Mid Level Questions
Q: What safety precautions do you take when operating a centrifuge?
Expected Answer: Should mention proper sample balancing, checking for tube integrity, using appropriate safety equipment, following speed limits, and proper cleanup procedures.
Q: How do you determine the right speed and time for centrifugation?
Expected Answer: Should explain how they consider sample type, required separation, protocol requirements, and manufacturer guidelines to set appropriate parameters.
Junior Level Questions
Q: What is the basic principle of centrifugation?
Expected Answer: Should explain in simple terms how spinning separates materials based on their weight, like separating blood components or concentrating samples.
Q: How do you properly balance samples in a centrifuge?
Expected Answer: Should describe placing tubes of equal weight opposite each other, using water or balance tubes when needed, and checking for proper balance before starting.
Experience Level Indicators
Junior (0-2 years)
- Basic centrifuge operation
- Sample preparation
- Safety protocols
- Record keeping
Mid (2-5 years)
- Multiple centrifuge type operation
- Protocol optimization
- Equipment maintenance
- Training newer staff
Senior (5+ years)
- Advanced troubleshooting
- Quality control management
- Protocol development
- Team supervision
Red Flags to Watch For
- Unfamiliarity with basic safety protocols
- No knowledge of proper sample handling
- Unable to explain basic maintenance procedures
- Lack of documentation experience
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